Additional file 2: Figure S1. of Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China

Comparison of the serological relationship of Calla lily chlorotic spot virus (CCSV), Tomato zonate spot virus (TZSV) and Tomato necrotic spot associated virus (TNSaV). The previously reported monoclonal antibodies MAb-CCSV-NP [24] (A and B) and MAb-WNSs [25] (C and D) were used to react with the crude leaf extracts of Nicotiana benthamiana plants separately infected with CCSV, TZSV, TNSaV or Watermelon silver mottle virus (WSMoV) in indirect enzyme-linked immunosorbent assay (A and C) and immunoblotting (B and D). The crude extract of a healthy N. benthamiana leaf (H) was used as the negative control. The plant ribulose bisphosphate carboxylase/oxygenase (rubisco) of Ponceau S staining is shown under immunoblotting to indicate the loading quantity. MAb-CCSV-NP was used at a 10−4 dilution. MAb-WNSs was used at a 10−3 dilution. (DOCX 124 kb)