Additional file 1: of Spherical Body Protein 2 truncated copy 11 as a specific Babesia bovis attenuation marker

Table S1. Primers used to amplify sbp2t7, sbp2t9 and sbp2t11 in Australian T and D strains. Sequence of primers designed and used to amplify target genes from cDNA as indicated in methods. Tm and amplicon size are indicated. Table S2. Primers used for quantitative PCR of sbp2t7, sbp2t9 and sbp2t11 in Australian T and D strains. The sequence of primers designed and used for qPCR of each gene analyzed as indicated in methods. Amplicons were validated by cloning and sequencing. Tm and amplicon size are indicated. Table S3. Nucleotide comparison of sbp2t7, sbp2t9 and sbp2t11 between Texas and Australian strains. Sbp2t7, sbp2t9 and sbp2t11 in T and Dixie Australian B. bovis strains were reported in GenBank with accession numbers MG430176, MG430177 and MG430178, respectively. These sequences were compared with the reference Texas strain of B. bovis by MacVector 12.0.5. Base pair (bp), nucleotide percent identity and amino acid percent identity are indicated. DOC. Table S4. Percentage identity between SBP2 and its 12 truncated proteins. Amino acid sequence alignment of SBP2 protein and its twelve truncated proteins (SBP2t) in Texas Babesia bovis strain were performed by MacVector 12.0.5. Identity scores are indicated in percentages. (DOCX 32 kb)