Additional file 1: of Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration

Figure S1. Monocyte infiltration occurs through retinal vasculature and the optic nerve head. (A) Low and high magnification views of retinal IHC sections from Arr1−/− mice before (0 h) and after 24 h of light exposure. Note the appearance of small round cells along the vitreal surface after 24 h. Scale bar 200 μm. (B) IHC from sections through the optic nerve head (ONH) of Arr1−/− retina. Infiltrating cells, many of which were Cd11b+ (red), clustered around the ONH and optic stalk. Vitreal Cd11b+ cells may include a population of hyalocytes. Scale bar 50 μm. (C) In vivo OCT imaging detected infiltrating cells in the vitreous (arrows), near the optic stalk and peripapillary region, in Arr1−/− but not wildtype mice. Similar results were seen in more than 6 other mice at this time point. (PNG 3160 kb)