Additional file 1: of Molecular characterization of irinotecan (SN-38) resistant human breast cancer cell lines

This file contains the following: Table S1: a table with FISH data of TOP1/CEN-20 and TOP2A/CEN-17, TOP1/CEN-20 analyses of 52 human breast cancer cell lines. Figure S1: correlations between TOP1 copynumbers from FISH and SNP analyses, and correlation between TOP1 copy numbers and gene expression, presentation of 9 breast cancer cell lines with regards to TOP1 copy numbers, ER and HER2 status and the sensitivity to SN-38. Figure S2: consistency between Top1 protein levels and TOP1 copy numbers, Top1 enzymatic activity in 9 breast cancer cell lines and the influence of SN-38 on Top1 enzymatic activity, dose–response of 9 breast cancer cell lines to SN-38 and IC50 values. Figure S3: MTT data demonstration that the the acquired and de novo SN-38 resitant cell lines are resistant when grown in the concentration of SN-38 at which the the development of resistance was terminated. Figure S4: Growth curves of the SN-38 resistant cell lines in comparison to their parental and DMSO controls. Figure S5: Cell cycle distribution of resistant cell lines in comparison to their respective DMSO controls. Figure S6: Sensitivity to SN-38 in docetaxel resistant breast cancer cell lines. Figure S7: Global expression analysis showing sample clustering, the number of differentially expressed genes between resistant and wild type DMSO control cell lines and Enrichment of Gene Ontology Molecular Function. Figure S8: Western blotting of Top2a in SN-38 resistant cell lines in comparison to their respective controls. Figure S9: Nannocapillary analysis of Top1 and β-actin in SN-38 resistant MCF7 and MCF7 control cells. Figure S10: Western blotting of ID3 in SN-38 resistant MDA-MB-231 and MCF-7 cells and their controls cells. (PDF 757 kb)