Additional file 1: Figure S1. of Human liver mesenchymal stem/progenitor cells inhibit hepatic stellate cell activation: in vitro and in vivo evaluation

Effect of ADHLSC on HSC plating efficiency. (A) The counting of floating and adhering HSC number after 24 hours of co-culture with ADHLSC showed an increase in the number of floating HSC concomitant with a decrease in the number of adhering HSC at an ADHLSC/HSC ratio of 1/100 (n = 4). (B) The counting of the number of floating and adhering HSC after 24 hours of incubation with ADHLSC conditioned medium showed similarly an increase in the % of floating HSC and a decrease in the % of adhering HSC, as compared with HSC incubated the same period of time with HSC conditioned medium (n = 4). C, Following annexin V–PI staining, no significant difference in cell death induction was noticed between HSC cultivated for 24 hours with ADHLSC or with HSC conditioned medium (n = 4). Results are expressed as mean ± standard error of the mean (SEM). ***Denotes a p value <0.001; ** p < 0.01; * p < 0.05. (PPT 271 kb)