Additional file 1: Figure S1. of Functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts

Tracheal tufts are shed in the fourth instar larval exuviae during pupation. (A) Bright-field image of a lateral view of fourth instar larval exuviae showing that the eighth abdominal segment trachea (black rectangle), including the tracheal trunks (T) and the tracheal tufts (TT), are shed during pupation. (B) Bright-field image of a dissected pupal dorsal abdomen, showing the dorsal longitudinal tracheal trunks (T) and the absence of the eighth abdominal segment tracheal tufts (circle). Directional arrows: A anterior, P posterior, D dorsal, V ventral, L lateral. (PDF 391 kb)