Additional file 1: Figure S1. of Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP

Mass spectrometry of tryptic peptides of YFP fusion proteins recovered from upper and lower bands of HES-1 expressed the HuEV-A vector. Figure S2. Mass spectrometry of tryptic peptides of YFP fusion proteins recovered from upper and lower bands of URI as in Figure S1. Figure S3. Standard curve of YFP amounts versus fluorescence reading (excitation 475 nm, emission 527 nm). Figure S4. a) HeLa cells were plated and transfected with Fugene-HD (Promega) in different size wells according with the parameters reported in the table. b) After lysis of the cells in each of the wells, the YFP fluorescence of 10 ml of solution used for IP was 2 measured. Figure S5. Tet-ON HeLa cells were transfected with the HuEV-A construct encoding the target protein with an N-terminal FLAG, YFP (Venus) and V5 tag under a tet-inducible promoter. (PDF 5435 kb)