Additional file 1: Figure S1. of Chitosan gel vaccine protects against tumour growth in an intracaecal mouse model of cancer by modulating systemic immune responses

Flow cytometry gating for identification of tumour immune cell infiltrates. Singlets were identified by gating with FSC- H versus FSC-A then SSC-H versus SSC-A. Live cells had low levels of Live/Dead dye. Lymphocytes and large cells were identified based on SSC-A and FSC-A. F4/80 expression was used to designate macrophages within the large cell gate and CD11c was used as an identifying marker of dendritic cells. CD19 was used to identify B cells within the lymphocyte gate and CD3 was used to identify T cells. CD8+ and CD4+ T cells were identified within the CD3 gate. (PDF 1568 kb)