Additional file 1: Figure S1. of A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53

Crystal structures of Mdm2 bound to p53 transactivation domain (17–29), nutlin, MI-773, and AMG232. (A) Mdm2 in magenta surface bound to the TAD1 of p53 (residues 17–29) in yellow ribbon with its three critical hydrophobic residues F19, W23, and L26 shown in sticks (PDB id: 1YCR) [17]. (B, C, D) Mdm2 in magenta surface bound to nutlin-3A (PDB id: 1RV1) [19], MI-773 (PDB id: 5TRF) [20], and AMG232 (PDB id: 4WT2) [21], respectively, in sticks. Images were generated using PyMOL (DeLano, W. L. The PyMOL Molecular Graphics System, DeLano Scientific, 2002). (EPS 6007 kb)