12943_2017_724_MOESM14_ESM.pdf (549.33 kB)
Additional file 14: of SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis
journal contribution
posted on 2017-10-11, 05:00 authored by Haihua Yuan, Rong Deng, Xian Zhao, Ran Chen, Guofang Hou, Hailong Zhang, Yanli Wang, Ming Xu, Bin Jiang, Jianxiu YuFig. S10. Hypoxia promotes KHSRP cytoplasmic localization. HeLa cells were stimulated by LY294002 (25 μM) for 0, 16 h before cells were harvested. (A) Nuclear and cytosolic fractions were extracted by the Nuclear/Cytosol fractionation kit. (B) Endogenous KHSRP was stained with the primary antibody anti-KHSRP (Rabbit), and then with the second antibody of Alexa Fluor 488 anti-rabbit. DAPI staining was to visualize the nucleus. All the images were taken by Nikon microscope, scale bar =25 μm (PDF 549 kb)