Additional file 11: Figure S11. of Glymphatic distribution of CSF-derived apoE into brain is isoform specific and suppressed during sleep deprivation

Cultured primary mouse choroid plexus epithelial cells secrete apoE. A) Representative apoE images from Western blots of the conditional medium from choroidal epithelial cells and astrocytes. B) Primary cultured choroidal epithelial cells (CP) produced similar levels of apoE as astrocytes (astro). Values are mean ± SEM, N = 4. C) Representative image of AQP1, a marker of choroid plexus epithelial cell, expression in the cultured primary mouse choroid plexus. Lack of significant immunolabeling of NeuN (neurons; D) and GFAP (astrocytes; E) in the cultured CP epithelial cells. F) Expression of GFAP in the cultured primary mouse astrocytes. DAPI (blue). (EPS 15343 kb)