Additional file 10: of Identification of the BRD1 interaction network and its impact on mental disorder risk

Upregulation of BRD1-S and BRD1-L. A QPCR measurement of BRD1 mRNA in BRD1-S and BRD1-L cells showed a ~24-fold higher BRD1 mRNA levels compared to HEK293T controls (*P <0.0001). Error bars indicate the standard error of the mean from n = 2 independent RNA samples. B Western blotting analysis using an antibody against endogenous BRD1 (BRD1 S/L) showed high levels of BRD1-S and BRD1-L in the stable cell lines compared to the expression level of BRD1 in HEK293T cells (293 T). D Expression array data showing log2 fold change values for probes that target BRD1 and seven housekeeping genes: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), Ubiquitin C (UBC), TATA box binding protein (TBP), hypoxanthine phosphoribosyltransferase 1 (HPRT1), Beta Actin (ACTB), Beta-2 microglobulin (B2M), and glucuronidase beta (GUSB). (PDF 76 kb)