Additional file 10: Figure S5. of The antiproliferative ELF2 isoform, ELF2B, induces apoptosis in vitro and perturbs early lymphocytic development in vivo

Reconstitution efficiency in ELF2+ retrogenic mice. A) Murine stem cell virus-based (MSCV) retroviral vector (pMIG) used for expressing HA-tagged Elf2 isoforms; primer sequences used for detecting specific isoform expression are indicated (arrowheads); a common 5’ primer within the HA-tag and 3’ primer able to detect all Elf2 isoforms were used. B) RT-qPCR of ectopic Elf2a isoform expression in the spleens of retrogenic mice after 3 months reconstitution. Analysis of GFP expression after 4 weeks in peripheral blood mononuclear cells: total C); T cell population D); B cell population E); and granulocytes F). Reconstitution efficiency in the haemopoietic compartment after 3 months. Data represents the mean ± SEM of 3 experiments each performed with 4–5 mice per experimental arm. Statistical analysis performed using Student’s t test (ns, not significant; *, p < 0.05; **, p < 0.01) (PDF 1218 kb)