Additional LANA expression is not sufficient for the early recruitment of EZH2 onto the KSHV genome.

(A) Time course analysis of LANA protein expression during de novo infection. (B) Time course ChIP analysis of LANA-binding to viral promoters during de novo infection. (C) Immunoblot analysis of LANA expression in empty lentiviral vector and 3xFLAG-LANA expressing lentivirus infected cells. (D) ChIP assays for binding of LANA and EZH2 to viral promoters in LANA-overexpressing cells during de novo KSHV infection.