Adaptability at a Protein−DNA Interface:  Re-engineering the Engrailed Homeodomain to Recognize an Unnatural Nucleotide

2004-07-07T00:00:00Z (GMT) by Matthew D. Simon Kevan M. Shokat
We explore the adaptability at a protein−DNA interface using phage display to re-engineer a homeodomain to specifically recognize a chemically synthesized, unnatural nucleotide. The engineered homeodomain preferentially binds to the modified DNA with affinity and specificity similar to those of natural homeodomains.