Adaptability at a Protein−DNA Interface:  Re-engineering the Engrailed
Homeodomain to Recognize an Unnatural Nucleotide

D. Simon, Matthew; Shokat, Kevan M.

doi:10.1021/ja048113w.s001

ja048113w_si_001.pdf (206.43 kB)

Adaptability at a Protein−DNA Interface: Re-engineering the Engrailed Homeodomain to Recognize an Unnatural Nucleotide

journal contribution

posted on 2004-07-07, 00:00 authored by Matthew D. Simon, Kevan M. Shokat

We explore the adaptability at a protein−DNA interface using phage display to re-engineer a homeodomain to specifically recognize a chemically synthesized, unnatural nucleotide. The engineered homeodomain preferentially binds to the modified DNA with affinity and specificity similar to those of natural homeodomains.