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A Novel Use of Gentamicin in the ROS-Mediated Sensitization of NCI-H460 Lung Cancer Cells to Various Anticancer Agents
journal contribution
posted on 2013-12-20, 00:00 authored by Michael F. Cuccarese, Amit Singh, Mansoor Amiji, George A. O’DohertyAminoglycosides
are broad-spectrum antibiotics that are used for the treatment of
severe Gram-negative and Gram-positive bacterial infections. While
bactericidal effects of aminoglycosides are due to binding to the
30S subunit of the bacterial ribosome, aminoglycosides can affect
protein synthesis, intracellular calcium levels, and levels of reactive
oxygen species (ROS) in eukaryotic cells. While aminoglycosides can
be cytotoxic at high concentrations, our results show that at much
lower doses, gentamicin can be implemented as a sensitizing agent
for the NSCLC cell line NCI-H460, increasing the efficacy of camptothecin,
digitoxin, and vinblastine in vitro. We have also
established that this sensitization is reliant on the ROS response
generated by gentamicin.