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A Microfluidic Diluter Based on Pulse Width Flow Modulation
journal contribution
posted on 2009-07-01, 00:00 authored by Alar Ainla, Irep Gözen, Owe Orwar, Aldo JesorkaWe demonstrate that pulse width flow modulation (PWFM) can be used to design fast, accurate, and precise multistage dilution modules for microfluidic devices. The PWFM stage unit presented here yields 10-fold dilution, but several PWFM stages can be connected in series to yield higher-order dilutions. We have combined two stages in a device thus capable of diluting up to 100-fold, and we have experimentally determined a set of rules that can be conveniently utilized to design multistage diluters. Microfabrication with resist-based molds yielded geometrical channel height variances of 7% (22.9(16) μm) with corresponding hydraulic resistance variances of ∼20%. Pulsing frequencies, channel lengths, and flow pressures can be chosen within a wide range to establish the desired diluter properties. Finally, we illustrate the benefits of on-chip dilution in an example application where we investigate the effect of the Ca2+ concentration on a phospholipid bilayer spreading from a membrane reservoir onto a SiO2 surface. This is one of many possible applications where flexible concentration control is desirable.
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channel lengthsphospholipid bilayerPWFM stagesMicrofluidic Diluterconcentration controlexample applicationpulse width flow modulationmembrane reservoirSiO 2 surfacemicrofluidic devicesdiluter propertiesdilution modulesPWFM stage unitresistance variancesPulse Width Flow ModulationWeflow pressureschannel height variances
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