AMPEROMETRIC STUDY OF IMMUNO-ION-SELECTIVE ELECTRODE RESPONSES USING BIOTIN-AZA-CROWN-6 IONOPHORE

The liquid-liquid electrochemistry of N-biotin-1-monoaza-crown-6 (BMAZC6) for the assisted transfer of potassium has been studied across the 1,2-dichloroethane-water interface by cyclic voltammetry. A model of the mechanism of the assisted K+ transfer by BMAZC6 is proposed. The influence of avidin in the aqueous phase on the assisted transfer of potassium is discussed when BMAZC6 is present in either the aqueous or the 1,2-dichloroethane phase. The mechanism of the interaction of avidin with the BMAZC6 is discussed. This assisted ion-transfer method is useful for the analytical determination of avidin down to 5 X 10(-7) M by differential pulse voltammetry.