Fig 7.tif (486 kB)
ACT and AVP additively induced astrocyte injury.
figure
posted on 2017-12-07, 18:58 authored by Jonghyuk Park, Takahiro Masaki, Yoshihiro Mezaki, Hiroshi Yokoyama, Mariko Nakamura, Haruka Maehashi, Takahiko J. Fujimi, Sabine S. Gouraud, Keisuke Nagatsuma, Madoka Nakagomi, Naofumi Kimura, Tomokazu Matsuura(A) Hoechst 33342 and PI double-staining of astrocytes. Cells were cultured for 48h in AVP-free medium supplemented with AVP, ACT, a combination of ACT and AVP, or a combination of ACT and AVP with OPC-21268. (B) The percentages of dead cells were calculated by determining the ratio of PI-stained cells to Hoechst-stained cells. (C) The expression of AQP4 mRNA in cells cultured under the same conditions as (A) was analyzed by a quantitative real-time RT-PCR. The bars indicate the mean ± standard error of the mean (n = 3). *p < 0.05, in comparison to control, # p < 0.05.
