ACBF5 is required for aNOP10 association and association of both proteins depends upon the ACA sequence

Copyright information:

Taken from "Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation"

Nucleic Acids Research 2005;33(10):3133-3144.

Published online 2 Jun 2005

PMCID:PMC1142404.

© The Author 2005. Published by Oxford University Press. All rights reserved

( and ) Identification by EMSA of the RNP complexes formed with the radiolabeled WT Pab91 sRNA (50 fmol) and various combinations of the core proteins (200 nM each). Incubation was at 65°C. The names of RNPs revealed by phosphorimager analysis are indicated. The percentage of sRNA present in each RNP as referred to the total RNA amount is indicated below each lane. ( and ) The same experiments as in panels A and B were repeated with the variant Pab91mtACA sRNA (Panel C), Pab91mtGA (Panel D) and Pab91mtGAmtACA (Panel D) sRNAs.