ACBF5 is required for aNOP10 association and association of both proteins depends upon the ACA sequence

<p><b>Copyright information:</b></p><p>Taken from "Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation"</p><p>Nucleic Acids Research 2005;33(10):3133-3144.</p><p>Published online 2 Jun 2005</p><p>PMCID:PMC1142404.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> ( and ) Identification by EMSA of the RNP complexes formed with the radiolabeled WT Pab91 sRNA (50 fmol) and various combinations of the core proteins (200 nM each). Incubation was at 65°C. The names of RNPs revealed by phosphorimager analysis are indicated. The percentage of sRNA present in each RNP as referred to the total RNA amount is indicated below each lane. ( and ) The same experiments as in panels A and B were repeated with the variant Pab91mtACA sRNA (Panel C), Pab91mtGA (Panel D) and Pab91mtGAmtACA (Panel D) sRNAs.