Gki630f2.jpg (44.17 kB)
ACBF5 is required for aNOP10 association and association of both proteins depends upon the ACA sequence
figure
posted on 2011-12-31, 00:01 authored by Bruno Charpentier, Sébastien Muller, Christiane BranlantCopyright information:
Taken from "Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation"
Nucleic Acids Research 2005;33(10):3133-3144.
Published online 2 Jun 2005
PMCID:PMC1142404.
© The Author 2005. Published by Oxford University Press. All rights reserved
( and ) Identification by EMSA of the RNP complexes formed with the radiolabeled WT Pab91 sRNA (50 fmol) and various combinations of the core proteins (200 nM each). Incubation was at 65°C. The names of RNPs revealed by phosphorimager analysis are indicated. The percentage of sRNA present in each RNP as referred to the total RNA amount is indicated below each lane. ( and ) The same experiments as in panels A and B were repeated with the variant Pab91mtACA sRNA (Panel C), Pab91mtGA (Panel D) and Pab91mtGAmtACA (Panel D) sRNAs.History
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