Fig 7.TIF (1.7 MB)
3-MA attenuates the protective effect of HU 308 on DSS-induced colitis in mice and the inhibitory of HU 308 on NLRP3 inflammasome activation in colon from DSS-induced colitis mice.
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posted on 2016-09-09, 04:30 authored by Ping Ke, Bo-Zong Shao, Zhe-Qi Xu, Wei Wei, Bin-Ze Han, Xiong-Wen Chen, Ding-Feng Su, Chong LiuC57BL/6 mice were treated with 3% DSS, DSS + HU 308 (1 mg/kg, i.p.) or DSS + HU 308 (1 mg/kg, i.p.) + 3-MA (10 mg/kg, i.p.) daily for 8 days. The control mice were given tap water. 3-MA significantly inhibited the beneficial effect of HU 308 on body weight loss (A), bloody stool score (B), colon length (C) and colon inflammation (D-E). n = 10 per group. **P<0.01 vs. the control group; #P<0.05 vs. the DSS group, ##P<0.01 vs. the DSS group; &P<0.05 vs. the DSS+HU 308 group, &&P<0.01 vs. the DSS+HU 308 group. (F) 3-MA also attenuated the inhibitory effect of HU 308 on Casp-1 p20/Casp-1 p45 ratio and proIL-1β in colon. n = 6 per group. **P<0.01 vs. vehicle; #P<0.05 vs. HU 308 group.
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cannabinoid receptor 2Cannabinoid Receptor 2 Ameliorates DSS-Induced ColitisMacrophages Activationnucleotide-binding domainCB 2R processCB 2RLPSprotein 3Inhibiting NLRP 3 InflammasomeSQSTMIL -1βattenuated autophagyNLRP 3 inflammasome activationHU 308 treatment attenuated DSS-induced colitis miceCB 2R KO miceproIL -1βDSS-induced inflammationcolon inflammationAtg 5macrophages attenuatedLC 3-II ratiowild-type miceCB 2R agonistCB 2R ameliorates inflammationAMPK-mTOR-P 70SCB 2R knockoutC 57BL miceKnockdown autophagy-related gene 524 hWT controlHU 308CB 2R ameliorates DSS-induced colitis
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