Relative mRNA concentrations of 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGCS)-2 in mouse tissues in response to fasting and PPARα.

Values represent means ± SD of relative mRNA concentrations of wildtype (WT) and PPARα knockout (KO) mice that were fed ad libitum or fasted for 48 h (n = 16 for liver, kidney, heart, small intestine and hypothalamus; n = 8 for pituitary gland, n = 6 for thyroid gland). Data were analyzed by two-way ANOVA. Classification factors were genotype, fasting, and the interaction between both factors. P-values revealed by two-way ANOVA are noted above the figures. Individual means of the treatment groups were compared by Tukey's test in case of variance homogeneity. In case of variance heterogeneity, as revealed by Levene's test, individual means were compared by Games Howell test. Horizontal brackets represent differences between groups in post-hoc comparison (**p<0.001, *p<0.05).