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Relationship between GFP fluorescence intensity and CAG repeat tract length.

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posted on 2014-11-25, 02:51 authored by Beatriz A. Santillan, Christopher Moye, David Mittelman, John H. Wilson

A. Isolation of cells from different parts of the distribution of fluorescence intensity. Induced GFP(CAG)89 cells were sorted according to the indicated gates, single cells were grown into colonies, and their CAG tracts were amplified by PCR and sequenced. B. Distribution of tract lengths in cells sorted by fluorescence intensity. C. Fluorescence intensity of cells with different CAG tract lengths.

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