qRT-PCR analyses of CDKN2A gene expression.

The indicated cell lines were infected with an AAV vector carrying a CDKN2A gene (H83Y) downstream of the CMV or the hACTB promoter. The cells were then cultured for two days without G418 (left) or for four weeks with G418 (right), and total RNA was extracted from each infectant, converted to cDNA, and used as a template in qRT-PCR. The expression level of CDKN2A was normalized to that of the GAPDH gene in each sample and shown relative to the data of the DLD-1 cell line obtained with the CMV promoter (mean ± s.e.m.; n = 3). An AAV vector carrying no CDKN2A gene was used as V.C. p-values were determined based on Scheffe’s post hoc test and denoted in the graph.