Figure_S1.tif (7.29 MB)
Figure S1 - Mice with Hypomorphic Expression of the Sodium-Phosphate Cotransporter PiT1/Slc20a1 Have an Unexpected Normal Bone Mineralization
figure
posted on 2013-06-13, 02:22 authored by Annabelle Bourgine, Paul Pilet, Sara Diouani, Sophie Sourice, Julie Lesoeur, Sarah Beck-Cormier, Solmaz Khoshniat, Pierre Weiss, Gérard Friedlander, Jérôme Guicheux, Laurent BeckVon Kossa and Goldner’s trichrome histological staining of femurs from PiT1+/+ and hypomorphic PiT1neo/neo mice from 1-day to 300-days of age.
Femurs from 1- to 300-day-old (P1 to P300) PiT1+/+ and PiT1neo/neo mice were fixed in paraformaldehyde and stained using Von Kossa (A) or Goldner’s Masson trichrome (B) staining method. Using Von Kossa staining, the mineral deposition is stained in black. The Goldner’s trichrome reveals the glycoaminoglycan matrix in blue-green. With these two staining, the osteoid is stained in red. Bar represents 200 µm.
(TIF)
