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Western blot analysis of the SH protein.

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posted on 2013-03-07, 09:22 authored by Miranda de Graaf, Sander Herfst, Jamil Aarbiou, Peter C. Burgers, Fatiha Zaaraoui-Boutahar, Maarten Bijl, Wilfred van IJcken, Eefje J. A. Schrauwen, Albert D. M. E. Osterhaus, Theo M. Luider, Bob J. Scholte, Ron A. M. Fouchier, Arno C. Andeweg

293T cells transfected with pCAGGS (lane 1), pCAGGS-SH (lane 2), Vero-118 cells infected with HMPV (lane 3) or HMPV ΔSH (lanes 4) and purified virions of HMPVΔSH (lane 5) and HMPV (lane 6) were analyzed on 12.5% polyacryamide gels. The SH protein was detected using rabbit serum raised against a mixture of petides representing aa 2–16 and 95–110 of the SH protein.

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