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Transfection of CSFV UTR RNA triggers DNA laddering.

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posted on 2014-02-12, 03:02 authored by Wei-Li Hsu, Chung-Lun Chen, Shi-Wei Huang, Chia-Chen Wu, I-Hsuan Chen, Muthukumar Nadar, Yin-Peng Su, Ching-Hsiu Tsai

Porcine kidney (PK-15) or rabbit kidney (RK-13) cells were mock transfected (lane 1), or transfected with in vitro transcribed luciferase RNA (lane 2), or chimeric luciferase-CSFV UTR RNA (5′UTR-Luc-3′UTR, lane 3) for 16 h. Total cellular DNA was harvested for the DNA laddering assay. M1, M2, and M were standard DNA markers with various size ranges.

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