Transfection of A549 and PC9 lung cancer cell lines with lentivirus for TGFβ1 rs1892073+29C or +29T.

(A,B) Fluorescence labeling indicated the transfection efficiency was >99% for both cell lines. (C,D) Western blot analysis confirmed that transfection with either +27C or +29T led to overexpression of TGFβ1 in both precursor and active forms. Con, control lentivirus construct; glyceraldehyde-3-phosphate dehydrogenase (GADPH) was used as a loading control.