The effects of GATA3 knockdown on the gene expression of Th2-1^st, Th2-4^th and memory Th2 cells.

The effects of GATA3 knockdown on effector Th2-1^st (A), Th2-4^th (B) and memory Th2 cells (C) were determined with qRT-PCR. The relative expression (GATA3/Control siRNA) levels are shown. The expression data were rank-ordered from genes with the highest to the lowest relative expression ratios (GATA3/Control siRNA) in Th2-1^st cells. The filled squares indicate genes that showed decreases in GATA3 dependency in Th2-memory cells compared with that observed in Th2-1^st cells. The open squares indicate genes that showed increases in GATA3 dependency in Th2-memory cells compared with that observed in Th2-1^st cells. The lower dashed line indicates 10^-1/2 and the upper dashed line indicates 10^1/2.