Fig_5.tif (2.79 MB)
The effect of TrkB receptor inhibitor K252a on neurite outgrowth.
figure
posted on 2015-03-25, 03:46 authored by Katsuya Ogata, Norihito Shintani, Atsuko Hayata-Takano, Toshihiko Kamo, Shintaro Higashi, Kaoru Seiriki, Hisae Momosaki, David Vaudry, Hubert Vaudry, Ludovic Galas, Atsushi Kasai, Kazuki Nagayasu, Takanobu Nakazawa, Ryota Hashimoto, Yukio Ago, Toshio Matsuda, Akemichi Baba, Hitoshi HashimotoPrimary hippocampal neurons were cultured with 10 nM PACAP or 2 nM BDNF in the presence or absence of 200 nM K252a for 3 DIV and double-immunostained for pNF and MAP2. Representative pNF- (red) and MAP2- (green) immunostained images of neurons (A), axon length (B), and dendrite length (C) were shown. Values represent mean ± SEM of 60 neurons from three independent experiments. **P < 0.01 vs. control, ##P < 0.01 vs. without K252a, two-way ANOVA followed by Tukey-Kramer test. Scale bar, 20 μm.
