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The average puromycin resistant (puror) colonies and repair pathway contribution for AARP HEK293FRT cells.

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posted on 2015-03-11, 02:43 authored by Maria E. Morales, Travis B. White, Vincent A. Streva, Cecily B. DeFreece, Dale J. Hedges, Prescott L. Deininger

a) S4 Fig includes the sequences of the specific Alu elements in each construct.

b) Colony numbers per 106 cells are reported with standard error.

c) Alu/Alu recombination percentage was calculated as the number of repair junctions containing an intact Alu as a proportion of total number of sequenced repair junctions.

d) NHEJ percentage was calculated as the number of repair junctions containing a non-intact Alu as a proportion of total number of sequenced repair junctions.

e) Data could not be calculated because PCR and sequence analysis of repair products was not performed for 0.7%-AARP HEK293FRT cells.

f) The average of independent experiments using two scrambled-sequence AARP HEK293FRT cell lines (Scr1-AARP and Scr2-AARP; S5 Fig).

The average puromycin resistant (puror) colonies and repair pathway contribution for AARP HEK293FRT cells.

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