Figure_2.tif (1.34 MB)
The N-terminal region of BAG6 is required for SGTA binding.
figure
posted on 2013-03-22, 23:53 authored by Pawel Leznicki, Quentin P. Roebuck, Lydia Wunderley, Anne Clancy, Ewelina M. Krysztofinska, Rivka L. Isaacson, Jim Warwicker, Blanche Schwappach, Stephen HighThe salt sensitive binding of full-length BAG6 (A) and selected deletion mutants (B to E) to SGTA was quantified by subtracting the signal recovered with immobilized BSA (non-specific) from that recovered with immobilized SGTA (specific), and expressing the SGTA bound fraction as a percentage of the signal obtained for the input. In each case it was the major translation product that was quantified by phosphorimaging (see arrow). The values presented for each BAG6 derivative (F) are derived from three independent experiments (n = 3) and indicate the standard deviation.
