The LD activity profiles of single and group-housed male and female flies.

Male and female control flies (w¹¹¹⁸/+) were aged, entrained and assayed between the ages of 1 to 4-weeks. Flies were assayed singly or in large group activity monitors (10 per vial) for 48 hours under standard 12-hr lights on, 12-hr lights off (LD) conditions. Yellow and black bars indicate day (8:00am to 8:00pm) and night (8:00pm to 8:00am) time periods, respectively. Activity is presented as an average per individual fly. (A-B) The average activity or actogram profiles of single-housed WT (w¹¹¹⁸/+) male and female flies, between 1 to 4-weeks of age. (C-D) The average activity profiles of group-housed male and female from 1 to 4-weeks of age. (E-F) The average activity levels of group-housed male and female flies (per fly) during day (ZT0-12), night (ZT12-24) and mid-dark (ZT15-21) time periods. * P ≤ 0.05, ** P ≤ 0.01 and *** P ≤ 0.001. See S2 Fig and S2 Table for additional information.