Figure_1.tif (476.68 kB)
Strategy for Duplex SmartAmp-based detection of SNP (c.309T>G) in the MDM2 gene.
figure
posted on 2013-04-03, 05:25 authored by Yasuaki Enokida, Kimihiro Shimizu, Jun Atsumi, Alexander Lezhava, Yuki Tanaka, Yasumasa Kimura, Takahiro Soma, Takeshi Hanami, Yuki Kawai, Kengo Usui, Yasuko Okano, Seiichi Kakegawa, Hiroomi Ogawa, Yohei Miyamae, Yohei Miyagi, Haruhiko Nakayama, Toshihisa Ishikawa, Yoshihide Hayashizaki, Izumi TakeyoshiA: The MDM2 gene is located on chromosome 12q14.3-q15, where SNP c.309T>G resides in intron 1. Annealing sites of the TP, FP, BP, OP, nBP, and oBP primers are shown in this schematic illustration. The size of each annealing site is numerically indicated in base (b) units. nBP, oBP(WT), and oBP(SNP) are designed to compete with each other to anneal with a site proximal to the SNP position. B: Horizontal arrows indicate annealing sites of primers. SNP (c.309T>G) is indicated by a vertical arrow.
