Fig_2.tif (3.37 MB)
Spastin-M1 co-localizes with the pre-LD marker Hpos.
figure
posted on 2015-04-13, 06:19 authored by Chrisovalantis Papadopoulos, Genny Orso, Giuseppe Mancuso, Marija Herholz, Sentiljana Gumeni, Nimesha Tadepalle, Christian Jüngst, Anne Tzschichholz, Astrid Schauss, Stefan Höning, Aleksandra Trifunovic, Andrea Daga, Elena I. RugarliCOS7 cells co-expressing mCherry (A), mCherry-spastin-M1 (B) or mCherry-spastin-M87 (C) with GFP-HPos were starved overnight (24h stv) or additionally incubated with OA (24h stv, 24h OA). Spastin-M1 is present in HPos positive puncta upon starvation, and forms rings together with HPos after OA loading. Spastin-M87 does not co-localize with pre-LDs or mature LDs. Images are individual Z-stacks. Enlargements of boxed areas are shown. Scale bar, 10 μm.
