Figure_7.tif (835.86 kB)
Somatodendritic and axonal uptake of exosomes.
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posted on 2013-07-09, 02:00 authored by Carsten Frühbeis, Dominik Fröhlich, Wen Ping Kuo, Jesa Amphornrat, Sebastian Thilemann, Aiman S. Saab, Frank Kirchhoff, Wiebke Möbius, Sandra Goebbels, Klaus-Armin Nave, Anja Schneider, Mikael Simons, Matthias Klugmann, Jacqueline Trotter, Eva-Maria Krämer-AlbersCN were cultured in microfluidic chambers and axons were allowed to grow through the microgrooves for 7 d. Exosomes isolated from PKH67 stained (A, B green) or AAV/MBP-Cre infected pOL (C, D) were applied to either the somatodendritic (A, C) or axonal (B, D) compartment of the device. Cre-containing exosomes were added to AAV/CBA-floxstop-hrGFP infected CN. hrGFP expression indicates Cre-mediated recombination (C, D green). CN were stained with Tuj1 (red) and nuclei with Dapi (blue). Scale bar, 20 µm. (E) Quantification of recombinant neurons located within 100 µm above the microgrooves. Exemplary pictures are depicted above the chart. Asterisk indicates the area of quantification. Error bars, SEM (n = 4).
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BiochemistrycytochemistryimmunocytochemistryorganellesmetabolismBiological transportNeurochemistryneuromodulationMacromolecular assembliesMolecular cell biologyCellular structuresSubcellular organellesCellular typesneuronsMembranes and sortingneuroscienceCellular neuroscienceMolecular neuroscienceneurotransmittersneurologyDemyelinating disordersMultiple sclerosisaxonaluptake
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