Semen leukocytes in macaques.

(A) Seminal concentrations of six pro-inflammatory molecules in normal and leukocytospermic animals. (B) Proportion of each studied leukocyte subset among total CD45⁺ cells. (C) Semen vRNA load in SIV⁺ macaques with normal (n = 11, blue circle) or leukocytospermic (n = 8, half plein black circles) semen. (D) Number of CD45⁺ events acquired by flow cytometry per sample since the total collected semen cells were analyzed, for control uninfected (n = 20), and SIV-infected macaques, at 10 and 14 dpi (n = 7) and during chronic infection. The dotted line represents the leukocytospermia threshold (10,000 CD45⁺ events acquired) (n = 13). (E) Proportion of each subset among total CD45⁺ events in SIV⁻ macaques (n = 12, blue circle) and SIV⁺ macaques during primary infection (n = 7, red triangle) and chronic infection (n = 9, red diamond). (F) Seminal concentrations of IP-10, RANTES and IL-8 in SIV⁻ macaques (n = 8 for IP-10 and n = 12 for RANTES and IL-8, blue circle) and SIV⁺ macaques during primary infection (n = 6 for IP-10 and n = 10 for RANTES and IL-8, red triangle) and chronic infection (n = 8 for IP-10 and n = 16 for RANTES and IL-8, red diamond).