Scheme of the experimental protocol for cell line establishment either fresh from surgery specimens ( = patient-derived cell line) of following xenografting in nu/nu mice ( = xenopatient-derived cell line).

Established cell lines were comprehensively characterized and routinely cyropreserved together with xenograft-tissue, immune cells, serum, and normal as well as primary tumor tissue in a biobank. This procedure leads to generation of individualized, patient-derived tumor models available for functional analyses.