Figure_1.tif (218.09 kB)
Schematic diagram of IRES-based and dual promoter lentiviral vectors.
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posted on 2014-12-12, 03:03 authored by Emanuela Chiarella, Giovanna Carrà, Stefania Scicchitano, Bruna Codispoti, Tiziana Mega, Michela Lupia, Daniela Pelaggi, Maria G. Marafioti, Annamaria Aloisio, Marco Giordano, Giovanna Nappo, Cristina B. Spoleti, Teresa Grillone, Emilia D. Giovannone, Raffaella Spina, Francesca Bernaudo, Malcolm A. S. Moore, Heather M. Bond, Maria Mesuraca, Giovanni MorroneThe expression cassettes of the lentiviruses used in this study are illustrated. The two IRES-containing vectors, FUIGW and UMG-LV11, differ for the position of the transgene and EGFP cDNA relative to the IRES element. In both viruses the transcription of this bicistronic unit is driven by the UBC promoter. The UMG-LV5 and UMG-LV6 vectors use independent promoters positioned back-to-back: UBC for the transgene and WASP (W) for EGFP. These dual-promoter vectors differ only for the orientation of the expression cassette. A short synthetic polyA signal, based on that of the human growth hormone gene, is downstream of the transcriptional unit in anti-sense orientation and is indicated by a diamond (♦).
