Figure_1.tif (345.93 kB)
SDS-PAGE analysis of the purification of scFv-IC16 via Ni-NTA and Aβ1-16 GB1 NHS sepharose affinity chromatography.
figure
posted on 2013-03-27, 10:35 authored by Silke Dornieden, Andreas Müller-Schiffmann, Heinrich Sticht, Nan Jiang, Yeliz Cinar, Michael Wördehoff, Carsten Korth, Susanne Aileen Funke, Dieter Willbold1 µl of the lysate and 15 µl of the purified fractions were applied to 16% polyacrylamide gels [56]. Lane 1: cell lysate, lane 2: eluate after affinity chromatography via Ni NTA–Agarose, lane 3: eluate after Aβ1-16-GB1-NHS-Sepharose affinity chromatography. Target protein is more than 99% pure after final purification step.
