S14161 inhibited platelet aggregation.

Gel-filtered human platelets (2.5×10⁸/mL) were preincubated for 10 minutes with different concentrations of S14161 (2.5 µM, 5 µM and 10 µM) or vehicle (DMSO). Platelet aggregation was initiated with collagen (2 µg/mL), thrombin (0.1 U/mL), U46619 (1 µM), or ADP (10 µM). The aggregation curves are the representatives of at least three individual experiments. Means ± standard errors of the mean of platelet aggregation percentage from three experiments are plotted in the bar charts; *P<0.05, **P<0.01 and ***P<0.001 as compared with control. N.S. means no significance (n = 3).