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Reporter gene analysis of DRB1 promoter VDRE.

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posted on 2013-02-21, 06:57 authored by Sreeram V. Ramagopalan, Narelle J. Maugeri, Lahiru Handunnetthi, Matthew R. Lincoln, Sarah-Michelle Orton, David A. Dyment, Gabriele C. DeLuca, Blanca M. Herrera, Michael J. Chao, A. Dessa Sadovnick, George C. Ebers, Julian C. Knight

Raji B cells were transiently transfected with pGL3 luciferase constructs as indicated together with pRL_TK to normalise luciferase activity. Open bars indicate resting cells, grey shaded bars results following stimulation of transfected cells with 1,25-dihydroxyvitamin D3. Mean+/−SD of three independent transient transfection experiments are shown, each performed in quadruplicate.

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