Regulation of Tcf3 in ApcNN ESCs is associated with histone modifications.

Schematic representation of mouse Tcf3 locus and the different amplicons (P1–P8) analyzed by QPCR in chromatin immunoprecipitation experiment. Chromatin was isolated from ApcNN and wild type ESCs and was immunoprecipitated with specific antibodies against the activating histone marks (H3K4me3 and H3Ac) and the repression histone marks (H3k27me3 and H3K9me3). The input DNA (chromatin before immunoprecipitation) and immunoprecipitated DNA was quantified by QPCR and using specific primers as described in materials and methods. Values from each amplicon were normalized to input chromatin and fold change was calculated relative to the corresponding negative region (P1). Bars represent n = 2±SD.