Rapamycin shows varied effects against seizures induced by kainic acid (i.p.).

(A) Mean seizure scores (±SEM) taken at 5-min intervals for 4 independent cohorts of mice treated with rapamycin (4.5 mg/kg) or vehicle for 6 h (N = 16 mice/group) (p = 0.55). (B) Latency to onset of seizure stage ≥2 for mice in panel A (p = 0.04, Mann-Whitney U test; bar represents group mean). (C) Number of 5-min time intervals in seizure stage ≥2 for mice in panel A (p = 0.14, Mann-Whitney U test). (D) Maximum seizure scores over the entire observation period (2 h) for mice in panel A (p = 0.66, Mann-Whitney U test). (E) Mean seizure scores (±SEM) for 3 consecutive days of rapamycin treatment (i.e., 75 h of rapamycin exposure) (N = 16) or vehicle (N = 20) (p = 0.0002; Bonferroni correction for multiple comparisons, p = 0.002). (F) Latency to onset of seizure stage ≥2 for mice in panel E (p = 0.31, Mann-Whitney U test). (G) Number of 5-min time intervals in seizure stage ≥2 for mice in panel E (p = 0.14, Mann-Whitney U test). (H) Maximum seizure scores over the entire observation period (2 h) for mice in panel E (p = 0.15, Mann-Whitney U test). In terms of body weight, there were no statistically significant differences between mice treated with vehicle or rapamycin at 6 h (19.2±0.41 vs. 19.0±0.38, respectively; p = 0.80), 48 h (19.2±0.48 vs. 18.9±0.41, respectively; p = 0.72), or 75 h (20.0±0.44 vs. 19.3±0.40, respectively; p = 0.21). *, p<0.04.