Quantification of serum semaphorin 3A in different forms AKI and diabetes in mouse.

Semaphorin 3A was quantified using ELISA kit as described in Methods. A. Circulating levels of semaphorin 3A before and different time after cisplatin administration. Cisplatin administration was significantly upregulated semaphorin 3A in the blood. *p<0.005 vs. 0 hr. B. Circulating levels of semaphorin 3A in sham operated (0 hr) and different time after reperfusion. Ischemia reperfusion rapidly downregulated circulating semaphorin 3A. *p<0.005 vs. 0 hr. Values are mean ± SEM. n = 4–6.