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Purification of tetrameric swine H1N1 and avian H5N1 rNAs.
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posted on 2015-08-17, 02:58 authored by Marua Prevato, Ilaria Ferlenghi, Alessandra Bonci, Yasushi Uematsu, Giulia Anselmi, Fabiola Giusti, Sylvie Bertholet, Francois Legay, John Laird Telford, Ethan C. Settembre, Domenico Maione, Roberta Cozzi(A) Avian H5N1 rNA purification by ion metal affinity chromatography; SDS-PAGE followed by Coomassie staining. MW: molecular weight marker (kDa); lane 1: pooled crude supernatants; lane 2: flow-through; lane 3: fraction eluted after washing with 10 mM imidazole; lane 4: fraction eluted after wash with 20 mM imidazole; lane 5: fraction eluted with 300 mM imidazole. (B) Gel filtration chromatogram of His-purified avian H5N1 rNA recorded at 280 nm wavelength. (C) SDS-PAGE followed by Coomassie staining of final purified, soluble, tetrameric swine H1N1 (lane 1) and avian H5N1 (lane 2) rNAs.Data shown are representative of at least three independent experiments.
