Figure_2.tif (2.08 MB)
Protein expression of TCF-4 isoforms J and K.
figure
posted on 2012-06-29, 01:38 authored by Hironori Koga, Orkhontuya Tsedensodnom, Yoshito Tomimaru, Evan J. Walker, Han Chu Lee, Kang Mo Kim, Hirohisa Yano, Jack R. Wands, Miran Kim(A) Organization of the “short” (A, B) and the “long” (J, K) isoforms; TCF-4B and J lack the SxxSS motif. (B) Confocal laser-scanning microscopy demonstrates nuclear localization of TCF-4J and TCF-4K (green). Nuclei are counterstained by DAPI. (C) Nuclear (Nuc)/cytoplasmic (Cyto) fractionation followed by immunoblot analysis confirms the nuclear localization of TCF-4J and K isoforms. Lamin (lamin A/C) and γ-tubulin were used as nuclear and cytoplasmic markers, respectively.
