posted on 2015-07-16, 03:26authored byEric P. Ratliff, Ruth E. Mauntz, Roxanne W. Kotzebue, Arysa Gonzalez, Madhulika Achal, Ayeh Barekat, Kaelyn A. Finley, Jonathan M. Sparhawk, James E. Robinson, Deron R. Herr, Greg L. Harris, William J. Joiner, Kim D. Finley
Sequential protein extracts were prepared from adult male fly heads at different ages (1-day to 4-weeks) and used for Western blot analyses of Ref(2)P and ubiquitinated proteins. The Triton-X Fraction contains the Triton X-100 soluble proteins, while the SDS Fraction identifies the Triton X-100 insoluble/SDS-soluble proteins that have taken on aggregate-like characteristics.