Figure_9.tif (4.29 MB)
PrPC co-immunolabeling with synaptophysin (left) and PSD95 (right).
figure
posted on 2013-09-10, 02:04 authored by Federica Agostini, Carlos G. Dotti, Azucena Pérez-Cañamás, Maria Dolores Ledesma, Federico Benetti, Giuseppe LegnameConfocal images of fully developed hippocampal primary neurons (21 DIV). Normal (above) and surface immunolabeling (below) of PrPC (green) coupled with synaptophysin and PSD95 (red). Antibodies used: D18 (10 µg/mL and 20 µg/mL in surface immunolabeling InPro Biotechnology, Inc, South San Francisco), mouse monoclonal anti Synaptophisin (1:100; SySy), mouse monoclonal anti PSD95 (1:100; Sigma).
Scale bar = 10 µm.
