Figure_5.tif (2.1 MB)
Plasmodium actin I–α-actin chimera forms long filaments.
figure
posted on 2014-04-17, 02:56 authored by Juha Vahokoski, Saligram Prabhakar Bhargav, Ambroise Desfosses, Maria Andreadaki, Esa-Pekka Kumpula, Silvia Muñico Martinez, Alexander Ignatev, Simone Lepper, Friedrich Frischknecht, Inga Sidén-Kiamos, Carsten Sachse, Inari Kursula(A) Electron cryo-micrograph of the chimera filaments. (B) Negatively stained chimera filaments. (C) The crystal structure of the chimera (blue) resembles that of wild-type actin I (red). The zoomed views show the differences in the D-loop around Tyr54 (above) and the C-terminal helix and the hydrophobic residues nearby, which are in the canonical orientation in the chimera, unlike in actin I (below).
